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儿童MDS造血前体细胞caspase-3 mRNA表达受IL-15的影响研究
稿件作者：程涵蓉;叶中绿;陈铭珍;
作者单位：深圳市人民医院
中文摘要：[摘要] 目的：探讨白细胞介素15（interleukin-15，IL-15）对骨髓增生异常综合征（myelodysplastic syndrome，MDS）造血细胞caspase-3 mRNA表达的影响，以探讨IL-15抑制MDS CD34+造血干/祖细胞凋亡的可能机制。方法：采用吸附单克隆抗体的免疫磁珠分离系统分离纯化18例MDS患者骨髓CD34+细胞，采用逆转录-聚合酶链式反应（reverse transcription-polymerase chain reaction，RT-PCR）检测caspase-3 mRNA表达水平，观察IL-15对它们的影响。结果：发现IL-15下调体外培养的MDS造血前体细胞cpp32基因mRNA的表达在0~100 ng/mL范围内呈剂量依赖性，其差异具有统计学意义；从d1~d8，随着IL-15作用时间延长，下调caspase-3基因mRNA表达的能力越强，其差异具有统计学意义。结论：IL-15可呈时间与剂量依赖性的下调体外培养的MDS造血前体细胞caspase-3基因mRNA的表达。IL-15下调MDS CD34+造血干/祖细胞caspase-3 mRNA的表达，可为其抑制该细胞凋亡的作用机制之一。
关键字词：白细胞介素15；骨髓增生异常综合征；造血干细胞；caspase-3
研究领域：儿科血液系统疾病
图文分类：R 725.5
基金支持：湛江市科技攻关项目;
稿件页码：
The Role of Interleukin-15 on Caspase-3 mRNA Expression of Hematopoietic Cells in Children with Myel
英文摘要：[Abatract] Objective: To investigate the effects of interleukin-15 (IL-15) on caspase-3 mRNA expression of hematopoietic cells in myelodysplastic syndromes (MDS) patients. Methods: CD34+ cells in bone marrow from 18 MDS patients were purified with an immunomagnetic beads sorting system. RT-PCR was used to detect the expressing level of caspase-3 mRNA, and the effect of IL-15 on them was observed in order to discuss the possible mechanism of IL-15 in suppressing apoptosis of hematopoietic precursors. Results: IL-15 could put down caspase-3 mRNA level of hematopoietic cells in myelodysplastic syndromes (MDS) in a dose and time–dependent manner. The difference had statistical significance. Conclusions: Decreasing the expressing level of caspase-3 mRNA could be one of the mechanism of IL-15 in suppressing apoptosis of hematopoietic precursors.
[Key words] Interleukin-15; Myelodysplastic Syndromes; Hemopoietic stem cell; Caspase-3
关键字词：interleukin-15; myelodysplastic syndromes; hemopoietic stem cell; caspase-3